Choice of fluorophore affects dynamic DNA nanostructures
نویسندگان
چکیده
Abstract The ability to dynamically remodel DNA origami structures or functional nanodevices is highly desired in the field of nanotechnology. Concomitantly, use fluorophores track and validate dynamics such DNA-based architectures commonplace often unavoidable. It therefore crucial be aware side effects popular fluorophores, which are exchanged without considering potential impact on system. Here, we show that choice fluorophore can strongly affect reconfiguration nanostructures. To this end, encapsulate a triple-stranded (tsDNA) into water-in-oil compartments functionalize their periphery with single-stranded handle (ssDNA). Thus, tsDNA bind unbind from by reversible opening triplex subsequent strand displacement. Using combination experiments, molecular (MD) simulations, reaction-diffusion modelling, demonstrate for 12 different combinations it possible alter even inhibit nanostructure formation—without changing sequence. Besides its immediate importance design pH-responsive switches labelling, our work presents strategy precisely tune energy landscape dynamic nanodevices.
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2021
ISSN: ['1362-4962', '1362-4954', '0305-1048']
DOI: https://doi.org/10.1093/nar/gkab201